Antimicrobial Activity of Iron Nanoparticles Synthesized using Bambusa vulgaris Leaves
J. Environ. Nanotechnol., Volume 15, No 1 (March 2026) pp. 243-248
Abstract
The current study synthesized iron nanoparticles (FeNPs) through a sustainable green synthesis method which utilized aqueous extract of Bambusa vulgaris leaves. This method offers environmentally friendly as well as economically viable benefits. UV-Visible spectroscopy was used to confirm FeNPs formation, stating a distinct peak of absorption at 278 nm. Fourier Transform Infrared Spectroscopy was performed to identify the functional groups present in the synthesised FeNPs. Scanning Electron Microscopy examined the morphology, revealing mostly spherical FeNPs with slight agglomeration due to high surface energy. Energy-Dispersive X-ray spectroscopy was used to determine the elemental composition confirming the presence of iron along with oxygen and chlorine, suggesting the phytochemical involvement in synthesis. X-ray Diffraction established crystalline nature of FeNPs at nanometric dimensions. In addition, the FeNPs revealed significant antibacterial activity against Escherichia coli (E. coli), Staphylococcus aureus, and Pseudomonas aeruginosa. The FeNPs showed inhibition zones of 14 mm against E. coli and 20 mm against S. aureus and P. aeruginosa which demonstrated equivalent effectiveness to the standard antibiotic amikacin. The study results demonstrate that Bambusa vulgaris functions as a potential biological source which can produce active antimicrobial FeNPs with superior properties.
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Reference
Batool, F., Iqbal, M. S., Khan, S. U. D., Khan, J., Ahmed, B. and Qadie, M. I., Biologically synthesized iron nanoparticles from Phoenix dactylifera and antibacterial activities, Sci Rep., 11(1), 22132 (2021).
https://doi.org/10.1038/s41598-021-01374-4
Brar, K. K., Magdouli, S., Othmani, A., Ghanei, J., Narisetty, V., Sindhu, R., Binod, P., Arivalagan, P., Kumar, M. A. and Pandey, A., Green route for recycling of low-cost waste resources for the biosynthesis of nanoparticles and nano-materials – A review, Environ Res., 207, 112202 (2022).
https://doi.org/10.1016/j.envres.2021.112202
Devi T. B. and Ahmaruzzaman M., Green synthesis of silver, copper and iron nanoparticles and wastewater applications, Green Met. Nanopart., 13, 441–466 (2018).
https://doi.org/10.1002/9781119418900
Gottimukkala, K. S. V., Green synthesis of iron nanoparticles using green tea leaves extract, J Nanomed Biotherapeutic Discov., 7, 151 (2017).
https://doi.org/10.4172/2155-983X.1000151
Kiiamov, A. G., Ivanova, A. G., Solodov, A. N., Cherosov, M. A., Tayurskii, D. A. and Khannanov, A., Determination of the Size of Complex Iron Oxide Nanoparticles Using Various Physical Experimental Methods, Coatings., 13(9), 1589 (2023).
https://doi.org/10.3390/coatings13091589
Kiwumulo, H. F., Muwonge, H., Ibingira, C., Lubwama, M. and Kirabira, J. B. and Ssekitoleko, R. T., Green synthesis and characterization of iron-oxide nanoparticles using Moringa oleifera, BMC Res Notes., 15(1), 149 (2022).
https://doi.org/10.1186/s13104-022-06039-7
Madani, M., Hosny, S., Alshangiti, D. M., Nady, N., Alkhursani, S. A., Alkhaldi, H., Al-Gahtany, S. A., Ghobashy, M. M. and Gaber, G. A., Green synthesis of nanoparticles for varied applications: green renewable resources and energy-efficient synthetic routes, Nanotechnol Rev., 11, 731–759 (2022).
https://doi.org/10.1515/ntrev-2022-0034
Manikandan, J., Rajesh, P. and Selvakumar, S. V. K., Green route synthesis of Fe₃O₄ nanoparticles and exploring its photochemical activity and antibacterial activity, J. Environ. Nanotechnol., 11(1), 01–05 (2022).
https://doi.org/10.13074/jent.2022.03.221448
Mareedu, T., Poiba, V. and Vangalapati, M., Green synthesis of iron nanoparticles using green and black tea leaf extracts, Mater Today: Proc., 42, 1498–1501 (2021).
https://doi.org/10.1016/j.matpr.2021.01.444
Mustapha, T., Misni, N., Ithnin, N. R., Daskum, A. M. and Unyah, N. Z., A review on plants and microorganisms mediated synthesis of silver nanoparticles, the role of plant metabolites and applications, Int. J. Environ. Res. Pub. Health., 19, 674 (2022).
https://doi.org/10.3390/ijerph19020674
Muthumari, P., Dhanalakshmi, J., Petchimuthu, M., Jeeva, M., Akash, R. M. Naveen, S. and Madhu, S., Synthesis of iron nanoparticles using Manilkara zapota leaves, AIP Conf. Proce., 3170(1), 030005 (2024).
https://doi.org/10.1063/5.0215797
Pandit, C., Roy, A., Ghotekar, S., Khusro, A., Islam, M. N., Emran, T. B., Lam, S. E. and Bradley, D. A., Biological agents for the synthesis of nanoparticles and their applications, J. King Saud. Univ. Sci., 34(3), 101869 (2022).
https://doi.org/10.1016/j.jksus.2022.101869
Raja, N. R., Prabha, A. and Helen, S. M., Green synthesis and antimicrobial activity of iron nanoparticles using Mangifera indica leaf extract, Int. J. Creat. Res. Thoughts., 6(1), 1020-1025(2018).
Sasidharan, J., Meenakshi, R. V. and Sureshkumar, P., Green synthesis, characterization and evaluation of in-vitro antioxidant and anti-diabetic activity of nanoparticles from a polyherbal formulation-Mehani, J. Environ. Nanotechol., 7(3), 51–59 (2018).
https://doi.org/10.13074/jent.2018.09.183316
Shaker, A. L., Alimardani V., Tamaddon A. M., Amani A. M. and Taghizadeh S., Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract, Heliyon., 7(2), e06159 (2021).
https://doi.org/10.1016/j.heliyon.2021.e06159
Vishalatchi, M., Kalaiselvi, V., Yasotha, P. and Blessymol, B., Eco-friendly synthesis of ferric oxide nanoparticles – antimicrobial activity, J. Environ. Nanotechnol., 11(3), 28–34 (2022).
https://doi.org/10.13074/jent.2022.09.223459
Vitta, Y., Figueroa, M., Calderon, M. and Ciangherotti, C., Synthesis of iron nanoparticles from Eucalyptus robusta extract and evaluation of antioxidant and antimicrobial activity, Mater. Sci. Energy Technol., 3, 97–103 (2020).
https://doi.org/10.1016/j.mset.2019.10.014
Zakariya, N. A., Majeed, S. and Jusof, W. H. W., Investigation of Antioxidant and antibacterial activity of iron oxide nanoparticles synthesized from aqueous extract of Penicillium spp, Sen Int., 3, 100164 (2022).
https://doi.org/10.1016/j.sintl.2022.100164
Zangeneh, M. M. and Zangeneh, A., Biosynthesis of iron nanoparticles using Allium eriophyllum extract, Appl. Organomet. Chem., 34(1), e5304 (2020).
