A Comparative Study of PEGylated and PVPylated Surface-functionalized Zinc Oxide Nanoparticles for Environmental Applications
J. Environ. Nanotechnol., Volume 14, No 3 (September 2025) pp. 234-244
Abstract
Zinc oxide (ZnO) nanoparticles possess notable antibacterial and photocatalytic properties, yet the lack of comparative studies on PEGylated and PVPylated functionalized ZnO limits understanding of how surface modifications influence their effectiveness. A comparative study was conducted on ZnO nanoparticles synthesized using polyethylene glycol (PEG-ZnO) and polyvinylpyrrolidone (PVP-ZnO) as stabilizing agents, with zinc nitrate as the precursor. The synthesized nanoparticles were characterized using UV–Visible spectroscopy, X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and particle size analysis. Antibacterial activity was evaluated against Escherichia coli (MTCC: 912), Bacillus subtilis (MTCC: 121), and Streptococcus pyogenes (MTCC: 1925) using the well diffusion method. Photocatalytic efficiency was assessed through the degradation of methyl orange under sunlight irradiation using 100 mg of catalyst. UV–Vis analysis confirmed the formation of ZnO nanoparticles with absorption peaks at 203 nm (PEG-ZnO) and 207 nm (PVP-ZnO). X-ray diffraction patterns revealed crystalline nature, while EDX analysis confirmed the elemental composition of ZnO. Scanning electron microscopy images showed spherical morphology with minimal aggregation. Particle size analysis indicated average size of 330 ± 80 nm (PEG-ZnO) and 679 ± 45 nm (PVP-ZnO). PEG-ZnO exhibited enhanced antibacterial activity, with inhibition zones of 16.66 ± 0.66 mm (E. coli), 16.00 ± 0.88 mm (B. subtilis), and 13.66 ± 2.08 mm (S. pyogenes) at 100 mg/mL concentration, significantly higher than PVP-ZnO and control (p < 0.001). In the degradation study, PEG-ZnO achieved 95.16% methyl orange degradation within 50 minutes, significantly outperforming PVP-ZnO, which showed 84.16% degradation over the same period. These findings suggest that PEG- and PVP-functionalized ZnO nanoparticles are promising candidates for biomedical applications and environmental remediation.
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Abu, N. S. M., Shah, S. S., Chaity, S. B., Biswas, B. K., Nahar, B., Aziz, M. A. and Hossain, M. Z., Jute stick extract assisted hydrothermal synthesis of zinc oxide nanoflakes and their enhanced photocatalytic and antibacterial efficacy, Arabian J. Chem., 15(11), 104265(2022).
https://doi.org/10.1016/J.ARABJC.2022.104265
Abubakar, H. L., Tijani, J. O., Abdulkareem, S. A., Mann, A. and Mustapha, S., A review on the applications of zinc tungstate (ZnWO₄) photocatalyst for wastewater treatment, Heliyon, 8(7), e09964(2022).
https://doi.org/10.1016/J.HELIYON.2022.E09964
Ahmad, W. and Kalra, D., Green synthesis, characterization and antimicrobial activities of ZnO nanoparticles using Euphorbia hirta leaf extract, J. King Saud Univ. Sci., 32(4), 2358–2364(2020).
https://doi.org/10.1016/j.jksus.2020.03.014
AlAbdulaal, T. H., Abdullah, W. and Yahia, I. S., Synthesizing and exploring the structural and optical properties of PVA/PVP/PEG polymeric sheet upon doping with nano nickel oxide (NiO) for CUT-OFF filters, J. Mater. Res. Technol., 27, 8308–8322(2023).
https://doi.org/10.1016/J.JMRT.2023.11.199
Arshad, M., Neelam, A., Haleem, N., Jamal, Y., Hina, K., Bilal, M., Shah, S. H., Hussain, I. and Zhang, Y., Synthesis and characterization of CMC/PVA/PVP composite microfiltration membrane, Desalin. Water Treat., 203, 70–79(2020).
https://doi.org/10.5004/DWT.2020.26227
Avinash, B., Ravikumar, C. R., Basavaraju, N., Abebe, B., Kumar, T. N., Manjula, S. N. and Murthy, H. C. A., Facile green synthesis of zinc oxide nanoparticles: Its photocatalytic and electrochemical sensor for the determination of paracetamol and D-glucose, Environ. Func. Mater., 2(2), 13–141(2023).
https://doi.org/10.1016/J.EFMAT.2024.01.002
Ayub, Y., Farrukh, M. A., Ben, K. S., Chebaane, S., Ghodhbani, R., Ismail, M. A., Ibrar, M., Munawar, T. and Allakhverdiev, S. I., In-situ synthesis of polyethylene glycol-assisted combined semiconductor metallic oxides nanocomposite for solar cell, supercapacitor, photocatalytic, and antibacterial applications, Ceram. Int., 51(23), 28342(2025).
https://doi.org/10.1016/J.CERAMINT.2025.06.177
Babaei, P., Safaei, G. J., Rashki, S. and Mahmoudi, K. A., Morphology modified by polyvinylpyrrolidone for enhanced antibacterial and catalytic execution of bioactive Ag/ZnO composites based on hydroxyapatite in the synthesis of O-Aminocarbonitriles, Ceram. Int., 49(14), 22826–22836(2023).
https://doi.org/10.1016/J.CERAMINT.2023.04.106
Bhardwaj, K. and Singh, A. K., Bio-waste and natural resource mediated eco-friendly synthesis of zinc oxide nanoparticles and their photocatalytic application against dyes contaminated water, Chem. Eng. J. Adv., 16, 100536(2023).
https://doi.org/10.1016/J.CEJA.2023.100536
Coll, S. C., Rubio, H. M., Ducrot, A., Norkute, E., Liu, X., Ebrahim, F. M., Smit, B., Thulstrup, P. W. and Hosta, R. L., A novel PEG-mediated approach to entrap hemoglobin (Hb) within ZIF-8 nanoparticles: Balancing crystalline structure, Hb content and functionality, Biomater. Adv., 163, 213953(2024).
https://doi.org/10.1016/J.BIOADV.2024.213953
De-Lucas, G. E., Leret, P., Monte, S. M., Reinosa, J. J., Enríquez, E., Del, C. A., Cañete, M., Menéndez, J., Fernández, J. F. and Rubio, M. F., ZnOnanoporous spheres with broad-spectrum antimicrobial activity by physicochemical interactions, ACS Appl. Nano Mater., 1(7), 3214–3225(2018).
https://doi.org/10.1021/ACSANM.8B00402
El-Boubbou, K., Lemine, O. M., Algessair, S., Madkhali, N., Al-Najar, B., AlMatri, E., Ali, R. and Henini, M., Preparation and characterization of various PVPylated divalent metal-doped ferrite nanoparticles for magnetic hyperthermia, RSC Adv., 14(22), 15664–15679(2024).
https://doi.org/10.1039/D4RA01600A
Endeshaw, S. B., Goddati, M., Lee, J., Aga, F. G., Tufa, L. T. and Sabir, F. K., ZnO/CuOnanocomposites for enhanced photocatalytic and antibacterial applications: A comparative study of synthesis methods, J. Sci. Adv. Mater. Dev., 10(3), 100898(2025).
https://doi.org/10.1016/J.JSAMD.2025.100898
Gurusamy, M., Sellavel, M. and Kuppuvelsamy, V. B., A sustainable green synthesis for photocatalytic and antibacterial activity of zinc oxide nanoparticles using Cucumismaderaspatanus leaf extract, Desalin. Water Treat., 319, 100457(2024).
https://doi.org/10.1016/J.DWT.2024.100457
Jailani, A., Hidzer, M. H., Firdaus, A. H. M., Sapuan, S. M., Zainudin, E. S., Atiqah, A., Wan Jaafar, W. M. and Suryanegara, L., Enhancing polyvinyl alcohol (PVA) nanocomposites: Key properties, applications and challenges in advanced engineering, Defence Technol., 01679(2025).
https://doi.org/10.1016/J.DT.2025.05.020
Kaur, H., Sharma, A., Anand, K., Panday, A., Tagotra, S., Kakran, S., Singh, A. K., Alam, M. W., Kumar, S., Bouzid, G., Dalal, J. and Singh, G., Green synthesis of ZnO nanoparticles using E. cardamomum and zinc nitrate precursor: A dual-functional material for water purification and antibacterial applications, RSC Adv., 15(21), 16742–16765(2025).
https://doi.org/10.1039/D5RA01469G
Khaleel, M. R., Hashim, F. S. and Alkhayatt, A. H. O., Impact of pH and ZnO NPs on the fabricated PVA-PVP/ZnOnanofibers: Morphology, absorption behavior, anticancer, and antibacterial activity, Ceram. Int., 50(20), 40161–40170(2024).
https://doi.org/10.1016/J.CERAMINT.2024.07.283
Kumari, A., Sharma, D., Sharma, V. and Singh, B., Synthesis and characterization of sterculia gum-zwitterionicsulfobetaine-PVP based network hydrogels for biomedical application, Hybrid Adv., 8, 100341(2025).
https://doi.org/10.1016/J.HYBADV.2024.100341
Lin, P. Y., Huang, Y. H., Chen, J. S., Sharma, R. K., Das, K., Sukul, U., Dey, G., Banerjee, P., Shinde, M., Taharia, M. and Chen, C. Y., Cost-effective microbial induced ZnO synthesis for building material: Antibacterial, photocatalytic and mechanical characteristics, Environ. Technol. Innov., 36, 103762(2024).
https://doi.org/10.1016/J.ETI.2024.103762
Liu, Y., Liu, Y., Li, X., Qian, Y., Lv, L. and Wang, Y., Fabrication and research of Mg(OH)₂/PCL/PVP nanofiber membranes loaded by antibacterial and biosafe Mg(OH)₂ nanoparticles, Polymer. Test., 112, 107635(2022).
https://doi.org/10.1016/J.POLYMERTESTING.2022.107635
Magar, M. H., Adole, V. A., Waghchaure, R. H. and Pawar, T. B., Efficient photocatalytic degradation of eosin blue dye and antibacterial study using nanostructured zinc oxide and nickel modified zinc oxide, Res. Chem., 4, 100537(2022).
https://doi.org/10.1016/J.RECHEM.2022.100537
Mezel, M., Alsous, M. B., Abbas, B., Aljoumaa, K., Assfour, B., Mousa, N. and Assaad, I., Multimode gradient-index fiber coated with a blend of PEG and PVP as a humidity sensor, Res. Optics., 16, 100697(2024).
https://doi.org/10.1016/J.RIO.2024.100697
Nazir, A., Akbar, A., Baghdadi, H. B., urRehman, S., Al-Abbad, E., Fatima, M., Iqbal, M., Tamam, N., Alwadai, N. and Abbas, M., Zinc oxide nanoparticles fabrication using Eriobotrya japonica leaves extract: Photocatalytic performance and antibacterial activity evaluation, Arabian J. Chem., 14(8), 103251(2021).
https://doi.org/10.1016/J.ARABJC.2021.103251
Nguyen, N. T. T., Phan, A. N. Q., Tran, T. V. and Nguyen, T. T. T., Antibacterial and photocatalytic activities of fruit-extract-mediated synthesized ZnO nanoparticles, Nanoscale Adv., 7(11), 3514–3527(2025).
https://doi.org/10.1039/D5NA00211G
Nisha, J. A., Anilkumar, P. and Preetha, S., Eco-friendly fabrication of ZnOnanocomposites using Lepidium didymium and polymer-assisted CTAB/PEG: A multifunctional approach for enhanced biomedical applications, J. Mol. Liq., 417, 126550(2025).
https://doi.org/10.1016/J.MOLLIQ.2024.126550
Panchami, H. R., Isloor, A. M., Vijesh, A. M. and Farnood, R., Innovative membrane engineering: Polyphenylsulfone/silver-doped zinc oxide for high-efficiency protein rejection, Curr. Res. Green Sustain. Chem., 10, 100460(2025).
https://doi.org/10.1016/J.CRGSC.2025.100460
Prabhu, A., Lamani, A. R., Shetty, S. J., Bhat, S. S., Bhat, A. R., Jayanna, H. S. and Gurumurthy, S. C., Biosynthesis of ZnO nanoparticles using Averrhoabilimbi fruit extract: Evaluation of photocatalytic and antibacterial activities, Inorg. Chem. Commun., 180, 114989(2025).
https://doi.org/10.1016/J.INOCHE.2025.114989
Raha, S. and Ahmaruzzaman, M., ZnO nanostructured materials and their potential applications: Progress, challenges and perspectives, Nanoscale Adv., 4(8), 1868–1925(2022).
https://doi.org/10.1039/D1NA00880C
Rutherford, D., Bařinková, M. Š., Jamatia, T., Šuly, P., Cvek, M. and Rezek, B., Capping agent control over the physicochemical and antibacterial properties of ZnO nanoparticles, Appl. Surf. Sci., 692, 162739(2025).
https://doi.org/10.1016/J.APSUSC.2025.162739
Sharma, R. K., Bandichhor, R., Mishra, V., Sharma, S., Yadav, S., Mehta, S., Arora, B., Rana, P., Dutta, S. and Solanki, K., Advanced metal oxide-based nanocatalysts for the oxidative synthesis of fine chemicals, Mater. Adv., 4(8), 1795–1830(2023).
https://doi.org/10.1039/D2MA00977C
Sheehab, N. T., Hashim, F. S., Al-Bermany, E., Obaid, A. N., Abdali, K. and Alkhayatt, A. H. O., Performance of TiO₂-SiC nanomaterials on morphology and sorption behavior of PVA-PEG-based nanocomposites for UV-applications and antibacterial efficacy, Nano-Struct. Nano-Obj., 42, 101479(2025).
https://doi.org/10.1016/j.nanoso.2025.101479
Uzoeto, H. O., Nkiru, A., Aisida, S. O. and Ezema, F. I., Enhanced microstructural properties and biocompatibility of zinc oxide nanoparticles by biogenic synthesis protocol for antibacterial applications, Hybrid Adv., 7, 100332(2024).
https://doi.org/10.1016/J.HYBADV.2024.100332
Villegas, F. A., Castellano, L. E., Vilchis, N. A. R., and Luque, P. A., Sustainable and environmentally friendly synthesis of ZnO semiconductor nanoparticles from Bauhinia forficata leaves extract and the study of their photocatalytic and antibacterial activity, Cleaner Mater., 13, 100268(2024).
