Green Synthesis, Characterization and Evaluation of In-vitro Antioxidant & Anti-diabetic Activity of Nanoparticles from a Polyherbal formulation-Mehani
J. Environ. Nanotechnol., Volume 7, No 3 (2018) pp. 51-59
Abstract
Green synthesis of silver and iron nanoparticles was carried out using Polyherbal formulation Mehani and characterized through UV-Visible absorption spectroscopy, FTIR, XRD and SEM respectively. The color change and strong peak at 465nm on UV-spec analysis initially conforms the synthesis of silver nanoparticles. Peaks were not obtained for iron nanoparticles since it is zero valent in nature. The FTIR study reveals the phytochemicals responsible for the reduction of silver and iron nanoparticles. The crystalline nature of the presence of silver and iron nanoparticles was confirmed by XRD analysis by comparing with standard JCPDS file No. 89-3722, 75-0033. Surface morphology and size were identified using SEM analysis and it was found to be 60-80nm, 40-60nm respectively. Total phenol and flavonoid content were also estimated for the synthesized nanoparticles and found to be 2.91 and 1.93 (AgNPs), 3.71 and 5.185 (FeNPs). Antioxidant activities like DPPH, Nitric oxide, H2O2 scavenging activities and reducing power assays were performed. Anti-diabetic activity was performed using alpha amylase inhibition assay and the percentage of inhibition was found to be 65.45% (AgNPs), 70.48% (FeNPs) and 73.87% for standard ascorbic acid. The result clearly indicates that FeNPs possesses better antioxidant and anti-diabetic activity that can be used for the efficientfor the management of hyperglycemia.
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Reference
Bagherzade, G., Tavakoli, M. M., Namaei, M. H., Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria, Asian Pac. J. Trop. Biomed., 7(3), 227–233 (2017).
https://doi.org/10.1016/j.apjtb.2016.12.014
Balayssac, S., Trefi, S., Gilard, V., Malet-Martino, M., Martino, R., Delsuc, M.-A., 2D and 3D DOSY 1H NMR, a useful tool for analysis of complex mixtures: Application to herbal drugs or dietary supplements for erectile dysfunction, J. Pharm. Biomed. Anal., 50(4), 602–612 (2009).
https://doi.org/10.1016/j.jpba.2008.10.034
BLOIS, M. S., Antioxidant Determinations by the Use of a Stable Free Radical, Nature, 181(4617), 1199–1200 (1958).
https://doi.org/10.1038/1811199a0
Chanda, S., Dave, R., In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties : An overview, African J. Microbiol. Res., 3(13), 981–996 (2009).
https://doi.org/10.1007/s13197-011-0276-5
Dewanjee, S., Das, A. K., Sahu, R., Gangopadhyay, M., Antidiabetic activity of Diospyros peregrina fruit: Effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes, Food Chem. Toxicol., 47(10), 2679–2685 (2009).
https://doi.org/10.1016/j.fct.2009.07.038
Giacco, F., Brownlee, M., Oxidative stress and diabetic complications., Circ. Res., 107(9), 1058–70 (2010).
https://doi.org/10.1161/CIRCRESAHA.110.223545
Jayaprakasha, G. K., Jaganmohan Rao, L., Sakariah, K. K., Antioxidant activities of flavidin in different in vitro model systems, Bioorg. Med. Chem., 12(19), 5141–5146 (2004).
https://doi.org/10.1016/j.bmc.2004.07.028
Kalia, A., Gauttam, V., Development of polyherbal antidiabetic formulation encapsulated in the phospholipids vesicle system, J. Adv. Pharm. Technol. Res., 4(2), 108 (2013).
https://doi.org/10.4103/2231-4040.111527
Kharissova, O. V., Dias, H. V. R., Kharisov, B. I., Pérez, B. O., Pérez, V. M. J., The greener synthesis of nanoparticles, Trends Biotechnol., 31(4), 240–248 (2013).
https://doi.org/10.1016/j.tibtech.2013.01.003
KIM, J.-S., KWON, C.-S., SON, K. H., Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid, Biosci. Biotechnol. Biochem,. 64(11), 2458–2461 (2000).
https://doi.org/10.1271/bbb.64.2458
KSV, G., Green Synthesis of Iron Nanoparticles Using Green Tea leaves Extract, J. Nanomedine Biotherapeutic Discov., 7(1), 1-4 (2017)
https://doi.org/10.4172/2155-983X.1000151
Lee, H. S., Antioxidative activity of browning reaction products isolated from storage-aged orange juice, J. Agric. Food Chem., 40(4), 550–552 (1992).
https://doi.org/10.1021/jf00016a004
Li, Z., Geng, Y.-N., Jiang, J.-D., Kong, W.-J., Antioxidant and Anti-Inflammatory Activities of Berberine in the Treatment of Diabetes Mellitus, Evidence-Based Complement. Altern. Med., 1, 1–12 (2014).
https://doi.org/10.1155/2014/289264
Lipinski, B., Pathophysiology of oxidative stress in diabetes mellitus., J. Diabetes Complication,s 15(4), 203–210 (2001).
https://doi.org/10.1016/s1056-8727(01)00143-x
Liu, H., Liu, X., Jia, L., Liu, Y., Yang, H., Wang, G., Xie, L., Insulin therapy restores impaired function and expression of P-glycoprotein in blood-brain barrier of experimental diabetes., Biochem. Pharmacol., 75(8), 1649–58 (2008).
https://doi.org/10.1016/j.bcp.2008.01.004
McDonald, S., Prenzler, P. D., Antolovich, M., Robards, K., Phenolic content and antioxidant activity of olive extracts, Food Chem., 73(1), 73–84 (2001).
https://doi.org/10.1016/S0308-8146(00)00288-0
Nair, S. S., Kavrekar, V., Mishra, A., In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts, Eur. J. Exp. Biol., 3(1), 128–132 (2013).
Oyaizu, M., Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine., Japanese J. Nutr. Diet,. 44(6), 307–315 (1986).
https://doi.org/10.5264/eiyogakuzashi.44.307
Patel, D., Prasad, S., Kumar, R., Hemalatha, S., An overview on antidiabetic medicinal plants having insulin mimetic property, Asian Pac. J. Trop. Biomed., 2(4), 320–330 (2012).
https://doi.org/10.1016/S2221-1691(12)60032-X
Sadeghi, B., Rostami, A., Momeni, S. S., Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity, Spectrochim. Acta Part A, Mol. Biomol. Spectrosc., 134, 326–332 (2015).
https://doi.org/10.1016/j.saa.2014.05.078
Zhishen, J., Mengcheng, T., Jianming, W., The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals, Food Chem., 64(4), 555–559 (1999).
