Welcome to JENT its Friday 19th of January 2018

Journal of Environmental Nanotechnology

(A Quarterly Peer-reviewed and Refereed International Journal)
ISSN(Print):2279-07 48; ISSN(Online):2319-5541
CODEN:JENOE2

Depletion of Antioxidants Vitamins and Oxidation of Low Density Lipoproteins observed among different workers with NIDDM Exposed to Vehicle Exhaust in the congested part of the CHENNAI city

Abstract

This study was carried out among NIDDM patients who were exposed to traffic smoke more than 10hrs/day. We have measured the level of Vitamins, Vitamin C, Superoxide dismutase (SOD), Catalase, Oxidised LDL, Total thiols, Total cholesterol, plasma blood glucose, Urea, Creatinine and Urine sugar. It was found that the level of vitamin E, C, Catalase, total thiols was found to be depleted but the level of Oxidised LDL, LPO, LOH was found to be elevated. SOD was found to be slightly elevated, Common biochemical parameters plasma blood glucose, urea, creatinine was found to be increased. Most of the persons who participated in our study were NIDDM patients with varying degrees of severity of complications. The patients case histories were recorded which consist of the age, the hours of exposure to traffic smoke, the duration of diabetes in years. Sixty non-smoking males also participated as control. No Gross variation was observed in Lipid profile samples.

Article Type: Research Article

Corresponding Author: V. N. Janakarajan 1  

Email: vnjn@hotmail.com

This article has not yet been cited.

V. N. Janakarajan 1*,  M. Rajendran 2,  Saravana balaji  3,  A. Ambika 4,  P. Sasikumar 5,  Radhakrishnan 6.  

1, 2. IRT Perundurai medical college, TN, India

3. RVS Dental college, Sulur, Coimbatore, TN, India

4, 5. Department of Biochemistry, Bharathiar university,Coimbatore, TN, India

6. Former Biochemist, Madras medical college (MMC), India

J. Environ. Nanotechnol. Volume 3, No.3 pp. 88-95
ISSN: 2279-0748 eISSN: 2319-5541
ENT 143090.pdf
Download Citation

Reference

Anitra, C., Carr, Ben Zhan Zhu, and Balz Frei., Potential Antiatherogenic Mechanism of Ascorbate (Vitamin C)and α –Tocopherol(Vitamin E)., Cir Res, 87, 349-354 (2000).

Barnes P. J., Karin M, Nuclear Factor- Kappa B :a pivotal transcription factor in chronic inflammatory disease,N Eng. J.Med, 336, 1066-1071 (1997).    

Brown Lee M., Advanced protein Glycation in diabetes and Ageing., Ann. Rev. Med 46, 223-234 (1995).

doi:10.1146/annurev.med.46.1.223

Brown Lee, M., Biochemistry and Molecular cell biology of diabetic complications.,Nature, 414, 813-820(2001).

doi:10.1038/414813a

Buege J. A. and Aust S. D.,Microsomal lipid peroxidation, Methods in Enzymol., 52, 302-310 (1978).

doi:10.1016/S0076-6879(78)52032-6

Burton, G. W., Ingold K. U., Auto oxidation of Biological molecules:The antioxidant activity of Vitamin E and related chain breaking phenolic antioxidants invitro., J. Am. Chem. So.c, 103 (21), 6472-6477 (1981).

doi:10.1021/ja00411a035

Dodge, J. T., Mitchell G.,and Hanahan D.J., The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes .,Arch Biochem Biophys, 100, 119-130 (1963).

doi:10.1016/0003-9861(63)90042-0

Friedewald, W. T, Levy R. I, Fredrickson DS., Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge, Clinical Chemistry, 18 (6), 499–502(1972).

Halliwell B.,Gutteridge, J. M. C., Free Radicals in Biology and Medicine.,Oxford Clarendron press 2nd Edition,Society for Experimental biology and Medicine., 206, 248-254(1989).              

 Hasan, H. M. and Fridovich I., Regulation of the synthesis of Superoxide dismutase in Escherichia Coli,Induction by Methyl Viologen., The journal of Biological chemistry., 252, 7667-7672(1977).       

Hol Voet P., Vanhaecke J., Janssens S.,Van de Werf P.,Collen D.,Oxidised LDL and Malondialdehyde modified LDL in patient with acute coronary syndrome and stable coronary artery disease,Circulation, 98, 1487-1494 (1998).

doi:10.1161/01.CIR.98.15.1487

Kokoglu, E., Aktuglu, G., and Belce A., Leukocyte superoxide dismutase levels in acute and chronic Leukemias., Leukemia Res.,13, 457-458(1989).

doi:10.1016/0145-2126(89)90166-5

Koster, J. F., Biemond, P., Swaak A. J., Intracellular and Extracellular sulphydryl levels in Rheumatoid arthritis,Ann Rheu Dis, 45, 44-46(1986).

doi:10.1136/ard.45.1.44

Koya, D., King, G. L., Protein kinase C activation and the development of diabetic complications., Diabetes, 47, 859-866(1998).

doi:10.2337/diabetes.47.6.859

Krinsky, N. I., Deneke, S. M.,The interaction of oxygen and oxygen radicals with carotenoids., JNCI,68, 205-210,(1982).       

Kyriakis JM.,Avruch J.,Sounding the alarm :Protein kinase cascades activated by stress and inflammation, J Biol chem, 271, 24313-24316 (1996).

doi:10.1074/jbc.271.40.24313

Lang P. D. and Schettler,G., In Schettle G and R Gross Arteriosclerose,Grundlageon-Diagnostik-Therapie,Deutscher Arzte verlag Gmbh,cologne, W.Germany (1985).      

Larson K, Creatinine assay by a reaction kinetic principle, Clin.chim.Acta:41, 209-211 (1972).

doi:10.1016/0009-8981(72)90513-X

Lowry, O. H., Rosebrough,N. J., Farr, A. L., and Randal, R. J., Protein measurement with folin phenols reagent.,J.Biol.Chem., 193, 265-275 (1951).

Luckey, T. D.,Venugopal B. and Hutcheson., Heavy metal Toxicity and Hormonology,Academic press New York, 9, 650-654(1975)      

Marsh, M. H.,Fungerhut, B. and Miller, H.etal., Automated and manual direct method for the determination of blood urea, Clin.chem., 11, 624-627(1965).

Marshall, S., Garvey, W. T., Traxinger, R. R., New insight into the metabolic regulations of insulin action and insulin resistance role of glucose and aminoacids,FASEB.J, 5, 3031-3036 (1991).  

Matsumoto, S., Matsuo, M., litaka, Y. and Niki E., Oxidation of Vitamin E model compound 2,2,5,7,8-Pentamethylene chroman-6-01,with tetrabutyl peroxyl radical.,Journal of the Chemical Societ.Chemical Communication, 14, 1076-1077 (1986).

doi:10.1039/c39860001076

McCormick, D. B. and Wright, L. D.,Vitamins and coenzymes,In methods in Enzymology, McCormick DB,Wright LD, Eds New York Academic., (1980).    

 Misra and Fridowich I., The role of superoxide anion in the Autooxidation of Epinephrine and a simple assay for superoxide dismutase., J. Biolchem, 247, 3170-3175(1972).

Mullarkey, C. J., Edelstein D. and Brown Lee M., Free radical generation by early glycation products: a mechanism for accelerated atherogenesis in Diabetes.,Biochem.Biophys.Res.Commun.,173, 932-939 (1990).

doi:10.1016/S0006-291X(05)80875-7

Procter H.,Reynold ES.,Free radicals and disease in man.,Physiol Chem Phys, 16, 175-195 (1984).

Rister and Baehner, The Alteration of Superoxide Dismutase,Catalase,Glutathione peroxidise,and NAD(P)H Cytochrome c Reductase in Guinea pig polymorpho nuclar leukocytes and Alveolar Macrophags during Hyperoxia, J Clin Invest,Nov, 58 (5), 1174-1184 (1976).    

 Rosen P., Nawroth, P. P., King G.,Moller W., Tritschler R. J., Packer L., The Role of oxidative stress in the onset and progression of diabetes and its complications a summary of Congress Series sponsored by UNESCO-MCBN,The American Diabetes Association and the German Diabetes society. Diabetes Metab Res Rev.,17, 189-212,(2001).

doi:10.1002/dmrr.196

Rosen P., Nawroth, P. P., King G., Moller W., Tritschler, R. J., Packer, L.,The Role of oxidative stress in the onset and progression of diabetes and its complications a summary of Congress Series sponsored by UNESCO-MCBN,The American Diabetes Associatiation and the German Diabetes society. Diabetes Metab Res Rev.,17, 189-212 (2001).

doi:10.1002/dmrr.196

Schwaiger, H, Weirich, H. G, Brunner, Rass P, Hirsch-Kauffmann M., Groner, Y, Schweiger, M., Radiation sensitivity of Down's syndrome fibroblasts might be due to overexpressed Cu/Zn-superoxide dismutase (EC 1.15.1.1). Eur. J. Cell Biol, triangle park NC (1978).  

 Sinha, A. K. Colorimetric assay of catalase., Anal Biochem.,vol 47, 389-394 (1972).

doi:10.1016/0003-2697(72)90132-7

Steinberg, D., Parthasarathy, S.,Carew, T. E., Khoo, J. C.,Witztum, J. L., Beyond cholesterol modification of low- density lipoprotein that increases its atherogenicity,New Engl J Med,320, 915-924 (1989).

Stevens, M. J., Obrosova, I., Feldman, E. L. and Greene D. A., The sorbitol- osmotic and sorbitol –redox hypothesis,In Diabetes Mellitus :A Fundamental and clinical Text., LeRoith D.,Taylor SI.,Olef Sky JM.,Eds.Philadelphia Lippincott Williams&Wilkins 2000, 972-983 (2000).             

Tsan, Z. L.,Chin,  N., Kiser, M. D., Bigler WN., Specific spectrophotometry of Ascorbic acid in serum or plasma by use of ascorbic acid oxidase.,Clin Chem: 28, 2225-2228 (1982).               

Varley, H.,Gowenlock, A. H. and Bell, M., Practical Clinical Biochemistry Fifth edition,Heinemann Professional Publishing Ltd., Wang,J and Yang,G, Colorimetric detection of glycosylated haemoglobin in humans, 5, 385-405(1984),

Wolff, S. P. and Dean, R. T., Glucose Autooxidation and protein modification the potential role of "Auto oxidative glycosylation''in diabetes mellitus., Bio chem. J., 245, 243-250 (1987).

Zhen-Yue Jiang, Alison, C. S.,Woolard, D. and Wolff, S. P., Hydrogen peroxide production during experimental protein glycation,Febs Lett., 268,69-71 (1990).

doi:10.1016/0014-5793(90)80974-N

>>