Welcome to JENT its Thursday 22nd of February 2018

Journal of Environmental Nanotechnology

(A Quarterly Peer-reviewed and Refereed International Journal)
ISSN(Print):2279-07 48; ISSN(Online):2319-5541

Identification of Callus Induction Potential of Nine Different Genotypes of Indica Rice


Callus induction potential of nine economically important indica rice genotypes of Tamil Nadu and neighboring areas was examined on different callus induction medium. Nine genotypes include Improved White Ponni(I W Ponni), BPT-5204, TKM-9, CR1009, ADT-39, ADT-43, KKL(R) -1, CO(R)-48 and Savitri/ Ponmani. These rice genotypes were having distinct useful agronomical trait like resistance to various pest, tolerance to salinity and drought conditions. Most of the indica rice genotypes having good grain quality and nutritional properties are susceptible to pest and diseases causing loss in annual yield. The yield could be enhanced by genetic improvement by rice transformation to enable them to withstand biotic and abiotic stress. First step towards this approach is to produce a pleuripotent cell mass, callus induced from scutellum of mature rice seeds. MS basal medium with various 2, 4 Dichlorophenoxy acetic acid (2, 4-D) concentrations (2mg/L, 2.5mg/L, 3mg/L, 4mg/L and 5 mg/L) was used for these studies. Four indica rice genotypes I. W. Ponni, ADT- 43, BPT-5204 and TKM-9 found to be potent for callus induction from scutellum of mature seed at the concentration of 2mg/l to 5 mg /l 2,4-D. The varieties ADT-39, KKL (1) R, CO(R) 48, CR1009 and Savitri/Ponmani were recalcitrant and no embryogenic calli was obtained on either of the 2, 4-D concentration. 34 % of healthy calli obtained from I W Ponni 20 % of healthy callus induction obtained from BPT-5204 and 10 % of healthy calli from each of the ADT-43 and TKM-9. These indica rice genotypes could be used for various plant science researches.

Article Type: Orginal Research Article

Corresponding Author: L. Shukla 2  

Email: sachin.dhmgk@gmail.com

This article has not yet been cited.

S.N. Dhamangaonkar 1,  L. Shukla 2*.  

1, 2. Department of Biotechnology, Pondicherry University, Puducherry, India.

J. Environ. Nanotechnol. Volume 2, No.2 (2013) pp. 44-52
ISSN: 2279-0748 eISSN: 2319-5541
Download Citation


Aananthi, N., Anandakumar, C.R., Ushakumari, R. and Shanthi, P., Regeneration study of some indica rice cultivars followed by Agrobacterium mediated transformation of
highly regenerable cultivar, Pusa Basmati 1, Electronic J Plant Breeding, 1(4), 1249-1256

Abe, T, and Futsuhara, Y., Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.), Theor Appl Genet., 72, 3-10 (1986).

Grewal, D., Gill, R. and Gosal, S.S., Factors enhancing induction of high frequency plant
regeneration from somatic embryos of Indica rice (Oryza sativa L.), J Biological Sciences,
5(6), 697-702 (2005).

Hiei, Y., Ohta, S., Komari, T. and Kumashiro, T., Efficient transformation of rice (Oryzasativa L.) mediated by Agrobacterium & sequence analysis of the boundaries of the T-DNA, Plant J., 6, 271-282(1994).

Jain, R.K., Effect of some factors on plant regeneration from Indica rice cells and
protoplasts - a review, Ind J Exp Biol., 35, 323- 331(1997).

Jaseela, F., Sumitha, V.R. and Nair, G.M., Somatic embryogenesis and plantlet regeneration in an agronomically important wild rice speciesOryza nivara, Asian J Biotechnol., 1(2), 74-78 (2009).

Karthikeyan, A., Shilpha, J., Karuntha Pandian, and Ramesh, M., Agrobacterium mediated
transformation of indica rice cv. ADT-43, Plant cell Tissue Organ Cult., 109, 153-165 (2012).

Khaleda, L. and Al-Forkan, M., Genotypic variability in callus induction and plant
regeneration through somatic embryogenesis of five deepwater rice (Oryza sativa L.)
cultivars of Bangladesh, African J Biotechnol., Vol. 5 (16), pp. 1435-1440, 17 (2006).

Kumari, A. and Bhat, R.S., Kuruvinashetti Agrobacterium - mediated Genetic
Tranformation of BPT-5204, a commercially grown Indica rice variety, Karnataka J Agrc
Sci., 20(4), 845-847 (2007).

Lin, Y.J. and Zhang, Q.F., Optimizing the tissue culture conditions for high efficiency
transformation of Indica rice, Plant Cell Rep., 23, 540-547 (2005).

Meera Shaikh,. Rice knowledge management portal (www.rkmp.co.in) (2012).

Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tabacco tissue cultures, Physiologia Plantarum, 15, 473-497 (1962).

Naqvi, S.M., Razia, S. and Rashid, H., Tissue culture studies in Oryza sativa L. cvs. Basmati 385 and Super Basmati, Pak J Bot., 37 (4), 823- 828 (2005).

Nono Carsono., and Tomohiko Yoshida., Identify cation of Callus Induction Potential of 15 Indonesian Rice Genotypes, Plant Prod Sci., 9(1), 65-70 (2006).

Pandey, S.K., Ramesh, B. and Gupta, P.K.S., Study on effect on genotype and culture medium on callus formation and plant regeneration in rice (Oryza sativa L.), Indian J Genet., 54, 293- 299 (1994).

Panjaitan, Siti Nor Akmar Abdulla, Maheran Abdul Aziz, Sariah Meon and Othman Omar, Somatic Embryogenesis from Scutellar Embryo of Oryza sativa L. var. MR219, Pertanika J Trop Agric Sci., 32 (2), 185 – 194 (2009).

Prasanna, A., Balakrishna Murthy, P., Deecaraman, M. and Sridhar, R., Optimization of Callus Induction and Regeneration in Swarna Masoori Rice Cultivar Short Title: in Vitro Somatic Embryogenesis of Swarna Masoori Rice Cultivar, Res J Agric and Biol Sciences, 6(6), 917-922 (2010).

Revathi., and Arumugam Pillai, M., In vitro callus induction in rice (Oryza sativa L.), Res in Plant Biol., 1(5), 13-15(2011).

Sahoo, k., Tripathi, A., Pareek, A., Sopory, S. and Sneh, L., Singla-Pareek., An improved
protocol for efficient transformation and regeneration of diverse indica rice cultivars,
Plant Methods, 7, 49 (2011).

Shankhdhar, D., Shankhdhar, S.C. and Pant, R.C., Development of somatic embryos in rice, Indian J Plant Physiol., 7(3), 211-214 (2002).

Shanthi, P., Jebaraj, S., Geetha, S. and Aananthi, N., Effect of growth regulators on callus
induction in Rice embryo culture, Electronic J Plant Breeding, 1(4), 1200-1207 (2010).

Shoeb, F., Yadav, J.S., Bajaj, S. and Rajam, M.V., Polyamines as biomarkers for plant
regeneration capacity :improvement of regeneration by modulation of polyamine
metabolism in different genotypes of indica rice, Plant Sci., 160, 1229-1235 (2001).

Sridevi, G., Dhandapani, M. and Veluthambi. K., Agrobacterium -mediated transformation of white ponni, a non basmati variety of indica rice (Oryza sativa L.), Current Sci., 88(1),
128-132 (2005).

Tam, D.M. and Lang, N.T., In vitro selection for salt tolerance in rice, Omonrice, 11, 68-73

Verma, D., Joshi, R., Shukla, A. and Kumar Pramod., Protocol for invitro Somatic Embryogenesis and regeneration of rice (Oryza sativa L.), Indian J Exp Biol., 49, 958-963 (2011).

Visarada, K.B.R.S., Sailaja, M. and Sarma, N.P., Effect of callus induction media on
morphology of embryogenic calli in rice genotypes, Biol Planta., 45, 495-502 (2002).http://dx.doi.org/10.1023/A:1022323221513